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91077C
Sigma
Insulin, Recombinant Human
dry powder, for research or for further manufacturing use
| Synonym: | Insulin sodium salt human |
| CAS Number: | 11061-68-0 |
| EC Number: | 234-279-7 |
| MDL number: | MFCD00131380 |
Description
| Application | Serum-free medium supplements such as insulin are essential for long-term growth of commonly used mammalian cell lines. When insulin is absent from media, cell may exhibit disturbances in morphology and growth rate. Insulin, recombinant human (rHu) is identical in function and structure to the native human sequence. A hormone consisting of two polypeptide chains, rHu Insulin's A-chain (21 amino acids) and B-chain (30 amino acids) are covalently linked by disulfide bonds between cysteine residues. This genetically engineered human insulin is a full sequence molecule producted by recombinant DNA technology in baker's yeast. |
| Biochem/physiol Actions | Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. |
Properties
| form | dry powder |
| potency | ≥27.5 units per mg |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | NWG |
References
| reference | Smith, L.F., Species variation in the amino acid sequence of insulin. Am. J. Med. 40, 662, (1966) |
| Kono, T., Insulin-sensitive glucose transport. Vitam. Horm. 7, 103, (1988) |
